Facilities & Resources
Facilities
生物和Oceanographic科学系设在里德医学院 Building, the Nelms Buildings, and the Oceanographic科学 Research Institute. 还有 Life 科学 Museum that includes an impressive collection of specimens.
全球网络赌博平台的Facilities有一个工作湿实验室与曝气系统，水族馆维护 Oceanographic和淡水生物，一种专门养殖淡水鱼的水产养殖化合物 水产养殖，以及各种分光光度计，离心机，培养箱和显微镜 for the use of students and faculty in laboratory and research settings.
Our newly renovated Anatomy & Physiology laboratories allow students to engage in 动手探索使用解剖模型，仿真软件，包括游标 physiological tools, and specimens. 新 additions include newly designed air control systems and storage solutions. Utilizing these spaces, students have the opportunity 探索身体从细胞层面到整个有机体，不断收获 an appreciation for how the systems work together.
We have the capacity to teach students how to culture mammalian cells, amplify DNA 使用聚合酶链反应，使用荧光显微镜计数细胞和 view subcellular structures, and measure antibodies and antigens via ELISA assays.
Shimadzu AXIMA Performance MALDI TOF/TOF mass spectrometer
Funded by the State of Florida through the St. Johns River Initiative
MALDI质谱仪可以分析各种分子(毒素、 碳水化合物、聚合物、代谢物、寡核苷酸、蛋白质、多肽、细菌、 等.) that are pure as well as in complex mixtures, including 环境al samples with minimal sample preparation.
How MALDI-TOF MS works: Analytes are crystallized in a matrix and then loaded into 仪器. After 仪器 obtains a high vacuum, the researcher shoots the sample with laser pulses, releasing the analytes into the gas phase as ions. An 施加电场，加速分子离子的运动 a vacuum tube and strike a detector at the end. The time it takes an ion to travel the vacuum tube and strike the detector is dependent on its mass and charge. 由此产生的 data are used in a variety of ways, depending on the application. The TOF/TOF feature 允许对分析物进行MS/MS分析，这是一种串联质谱技术 仪器中的分子与氦原子破碎，提供结构 信息.
AXIMA-iDPlus:全球网络赌博平台的MALDI-TOF/TOF质谱仪配置并结合 SARAMIS软件和数据库，以识别各种细菌，酵母，霉菌， 和孢子. This technique relies on producing mass “fingerprints” of the microorganism and comparing it to the extensive database.
Features of 仪器:
- Mass range 1-80kDa in reflectron mode; 1-500kDa in linear mode
- Mass range 1-500kDa in linear mode; 1-80kDa in reflectron mode
- Mass resolution 20000 FWHM in reflectron mode; 5000 FWHM in linear mode
- Mass accuracy <5ppm in reflectron mode
- Sensitivity 250 amol in reflectron mode
- High energy MS/MS using helium collision gas to fragment molecules
- structural 信息 for the molecule
- amino acid sequences can be obtained from peptides
- 基于MASCOT数据库搜索集成的肽质指纹图谱蛋白质识别
- SARAMIS software and database for microorganism identification
学生和教师使用该仪器来识别和表征蛋白质、毒素、 and bacteria by analyzing intact proteins, peptides from digested proteins, algal toxins from local waters, and bacteria from local waters and oysters.
观看两个视频 of how some of our students have used the mass spectrometer which were filmed at the Science on the St. Johns community event at the JU Oceanographic科学 Research Institute:
Bio-Rad CFX96 Touch Real-Time PCR Instrument
Funded by the State of Florida through the JU EPIC program
Bio-Rad CFX96仪器用于进行聚合酶链反应(PCR); which is a molecular biology technique that copies and amplifies specific targets DNA的. The real-time feature means that the concentrations of specific genes can 在反应发生时(实时)进行监测的能力。.
How real-time PCR works:
聚合酶链反应使用DNA复制酶(Taq聚合酶)进行合成 copies of target DNA. This technique is the basis for DNA analysis of crime scenes 嫌疑是，从生物材料(血液)中扩增出短串联重复序列， saliva, semen, 等.) and the resulting data are compared. Real-time PCR additionally 当增加量时，加入引起荧光增加的分子 DNA的 are copied.
Features of 仪器:
- Ten orders of magnitude dynamic range
- Melting curve analysis
- 热梯度
- Three targets with different fluorophores can be analyzed simultaneously
- Takes 96 well plates, individual tubes, or strips
学生和教师使用该仪器来分析人类的Alu重复序列，以识别 细菌，比如 霍乱弧菌 (causes cholera) and Staphylococcus aureus (causes staph infections), and perform conventional (dark) PCR with subsequent analysis via agarose gel electrophoresis. Student projects can cover a range of target DNA.
FlowCam 8400
Donated by an anonymous donor
The FlowCam 8400 is a dynamic particle imaging and analysis system, which combines 流式细胞仪和显微相机具有计算机识别，计数， and biometric measurement capabilities.
Features of 仪器:
- Counts, captures, and saves images of particles
- Quickly (6 minutes) processes a 1 mL sample <100 µm particulates
- 测量和收集大小、形状、荧光和浓度统计信息(通常是 up to 10,000 images/minute)
- Identifies and characterizes organisms based on fluorescence
- Calculates biovolumes, concentrations, and community size distributions
- Sorts, filters, and classifies organisms with semi-automated imaging software
学生和教师可以使用FlowCam成像颗粒，范围从3um到1mm in size, from both aquatic and sedimentary field samples. The images and accompanying 数据可用于有害藻华监测、幼虫种群等目的 生态学研究，微塑料计数，重建历史天气和洋流 patterns (through analysis of sediment core particulates), and identifying cryptic species when used in conjunction with fluorescing probes. The FlowCam can also be 用于受控的实验室实验，以监测生长速率和确定存活率 thresholds under various parameters.
Biotage SP1 Flash Chromatograph
博士捐赠. 拉里·萨顿
Biotage SP1是一种闪光液相色谱系统，可以净化有机化合物 in an accelerated manner.
Features of this instrument:
- Window-based LCD touch screen interface
- Solvent and waste monitoring
- Variable wavelength detector (200 to 320nm)
学生和教师可以使用Biotage SP1快速色谱仪进行分离 以及有机化合物、铁载体、抗生素等化学物质的提纯 化合物.
Bio-Rad iMark Microplate Absorbance Reader
Funded by the State of Florida through the JU EPIC program
Features of 仪器:
- 400 nm – 750 nm wavelength range; dual wavelength readings
- Takes 96 well plates or strips
- 动力学分析
- 内置振动器
Students and faculty can use this instrument for ELISA analysis, bacterial growth curves, protein assays, enzyme assays for kinetics, and more.
SpectraMax Plus 385 Microplate Reader
博士捐赠. 拉里·萨顿
The SpectraMax plus can run as both a standard spectrophotometer and a microplate 读者.
Features of this instrument:
- Reads one sample or up to 384 reads in a single microplate.
- Temperature control and shaking capabilities
- Kinetic measurements
- Nucleic Acid and protein quantitation
Students and faculty can use the SpectraMax Plus 384 microplate 读者 for ELISA, Nucleic Acid quantitation, enzyme assays, protein quantitation, bacterial growth, antibiotic assays, and more.
GE AKTA Start Protein Purification System
博士捐赠. 拉里·萨顿
AKTA蛋白纯化系统是一种快速蛋白液相色谱系统 (FPLC).
Features of this instrument:
- Touchscreen display and real time monitoring
- Quick start methods and templates for common purification techniques
- Runs in either manual or automated modes
- Fraction collection
- UNICORN control software
Students and faculty can use the FPLC to analyze and purify proteins.
Genscript eStain L1
博士捐赠. 拉里·萨顿
The Genscript eStain L1 is a PAGE gel staining system.
Features of this instrument
- Has a sensitivity of ≤ 12.5 ng蛋白质
- Stains and destains in 10 minutes
教师 and student can use the eStain.to stain/destain two protein PAGE gels
生物安全柜
Funded by the State of Florida through the JU EPIC program
微生物生物安全柜用于学生和教师在无菌环境中工作 环境. Uses include handling bacteria, including pathogens, and preparing samples 用于PCR分析.
Leica DM IRB Inverted Fluorescent Microscope
Funded by the National Science Foundation
学生和教师已经使用这种显微镜来检测细胞中的特定蛋白质 using immuno-labeling, follow macrophage activity, determine and quantify live and dead bacteria, and visualize photosynthetic cells and subcellular components.
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Oceanographic Equipment
RBRduo3 CTDO2 (Conductivity, Temperature, Depths, Dissolved Oxygen) sensor
A CTD is one of the most common instruments in Physical Oceanography that measures
how temperature and salinity change in relation to depth.
Features of the instrument:
- 240M readings Standard sampling up to 1Hz
- Up to 32Hz sampling
- USB-C下载
- Real time communications
- 捻激活
- wi - fi准备好了
学生和教师可以在船舷、码头或停泊处使用CTD， 为了估计由密度变化引起的水密度和洋流， chemical and biological processes, find areas of upwelling and sediment movement, 计算声速并发现可能影响鱼类数量的低氧区域.
Pathfinder DVL Acoustic Doppler Current Profiler (ADCP)
ADCP是通过使用声波ping来测量水的运动.
声音由仪器发出，在水中传播，并在水中散射
and reflected back by suspended particles. The change in sound waves caused by this,
allows us to calculate the speed with which the water is moving.
Features of 仪器:
- Small size and weight allows the pathfinder to be installed on board the smallest vehicles or on small floating platforms.
- 相控阵:独特的相控阵传感器设计提供了更高的位置精度 at a reduced size, eliminates the need for speed of sound correction, and reduces susceptibility to biofouling.
- 灵活的设计:独立或远程头选项，可满足您的独特
车辆的需求.
Profiling capability. - Ethernet Compatible: Plug-n-play with today’s interfaces.
- 扩展范围:新的可选XRT(扩展范围跟踪)提供高达60%的增长 in bottom tracking range
Students and faculty can use the ADCP to measure the flow of the St. 约翰河和 以及水流在一天中不同时间、不同地区的变化， and during different conditions.
Aquarium Facilities
The Oceanographic科学 Research Institute houses the wetlab complete with aquarium and aquaculture tanks for freshwater and seawater projects.
Photos: Francisco Ortiz
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船只
The Oceanographic科学 Research Institute and Department of 生物学 and Oceanographic科学 have a number of vessels available for student use. 它们包括:
新! Jon Boat donated by North Florida Marine Association
18 ft. Flat Bottom Jon Boat
17 ft. 尖吻鲭鲨乱跑
RV海豚，25英尺. 波士顿的捕鲸船
痂attical, 31 ft. 喷泉
RV 拉金, 48英尺. Floating Classroom Pontoon Boat
For daily rates, please contact Director of the MSRI, Dr. 怀特: qwhite@stonecrusherestate.com.
Resources
OCEARCH at JU Scholarly Research
OCEARCH is a nonprofit with a focus on generating critical scientific data related 追踪(或遥测)和主要Oceanographic物种的生物学研究，包括 大白鲨和虎鲨，同时在该地区继续努力 of conservation and education, all on a measurable yet global scale. OCEARCH股票 real-time scientific data through its Global Shark Tracker and leverages that data 让学生参与互动课程计划，激励下一代 explorers, scientists, and stewards of the ocean.
Ocean Observatory Initiative
美国国家科学基金会的Oceanographic观测倡议(OOI)已经建成 一个持续的Oceanographic测量网络，用来研究气候变化、Oceanographic环流、 生态系统动力学、海气交换、海底过程和板块尺度地球动力学. 该项目还投资通过一系列节目向公众提供这些数据 基于网络的软件工具，将数据驱动的活动与实时数据连接起来 collected by the OOI.
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